Enzyme Kinetics: Summary
The answers to the ConcepTests are given below and will open in a separate window.
Key points from this module:
- An enzyme is a biological catalyst that increases the rate of reaction by decreasing the activation energy without being physically altered itself.
- For competitive inhibition, the inhibitor binds with the enzyme.
- For uncompetitive inhibition, the inhibitor binds with the enzyme-substrate complex.
- For non-competitive (or mixed) inhibition, the inhibitor and substrate bind with the enzyme at different sites.
- Increasing the inhibitor concentration decreases the rate of reaction.
From studying this module, you should now be able to:
- Derive Michaelis-Menten Kinetics.
- Identify what type of inhibition is occurring in an enzyme reaction.
- Evaluate how enzyme inhibition will alter the extent of a reaction.
- Determine kinetic parameters from rate data for an enzyme reaction mechanism.
Prepared by Ryan Dilbeck and John Falconer, Department of Chemical and Biological Engineering, University of Colorado Boulder