Enzyme Kinetics: Summary

The answers to the ConcepTests are given below and will open in a separate window. 
Key points from this module:
  1. An enzyme is a biological catalyst that increases the rate of reaction by decreasing the activation energy without being physically altered itself.
  2. For competitive inhibition, the inhibitor binds with the enzyme. 
  3. For uncompetitive inhibition, the inhibitor binds with the enzyme-substrate complex. 
  4. For non-competitive (or mixed) inhibition, the inhibitor and substrate bind with the enzyme at different sites.
  5. Increasing the inhibitor concentration decreases the rate of reaction.
From studying this module, you should now be able to:
  • Derive Michaelis-Menten Kinetics.
  • Identify what type of inhibition is occurring in an enzyme reaction.
  • Evaluate how enzyme inhibition will alter the extent of a reaction.
  • Determine kinetic parameters from rate data for an enzyme reaction mechanism.
Prepared by Ryan Dilbeck and John Falconer, Department of Chemical and Biological Engineering, University of Colorado Boulder